View zarr file using MoBIE and BDV

Description

This section shows how to view ome.zarr files in Fiji using BigDataViewer and MoBIE.

We show:

  • how to install the required dependencies

  • how to view an ome.zarr file stored in S3 using the User Interface

  • how to view an ome.zarr file stored in S3 using the scripting editor of Fiji.

Setup

  • Install Fiji on a local machine.

  • BigDataViewer is already included in Fiji.

  • To install MoBIE, go to Help>Update...

  • In the ImageJ updater dialog, click on Manage update sites.

  • The MoBIE` should already be listed. Select it and click Close.

  • Click Apply changes to install it.

  • If MoBIE` is not listed:
    • Click Add update site.

    • Enter for the name MoBIE (so you can identify it) and for the URL: https://sites.imagej.net/MoBIE/. See How to install an update site.

    • Click Apply changes to install it.

  • Restart Fiji.

Resources

Step-by-step

Opening an ome.zarr file in BigDataViewer

  1. Launch Fiji.

  2. Go to Plugins > BigDataViewer > HDF5/N5/Zarr/OME-NGFF Viewer

  3. A dialog pops up.

  4. In the text field, you can click Browse to open local data or enter the desired URL e.g.

https://uk1s3.embassy.ebi.ac.uk/idr/zarr/v0.4/idr0062A/6001240.zarr

  1. If you Click the OK` button tbis will open the image in BigDataViewer. You can also click the Detect datasets button to inspect the content of the zarr file, such as individual pyramid arrays and labels. You can select multiple items, for example to open the (multiscales) image under labels alongside the parent (multiscales) image.

  2. When the image is displayed in the BigDataViewer, select the dialog and press P to display the rendering controls.

  3. Modify the settings as you see fit.

Opening an ome.zarr file in MoBIE

  1. Launch Fiji.

  2. Go to Plugins > MoBIE > Open > Open OME-Zarr…

  3. A dialog pops up.

  4. In the text field, enter the desired URL e.g.

https://uk1s3.embassy.ebi.ac.uk/idr/zarr/v0.4/idr0062A/6001240.zarr

  1. Click the OK button.