View zarr file using MoBIE and BDV
Description
This section shows how to view ome.zarr files in Fiji using BigDataViewer and MoBIE.
We show:
how to install the required dependencies
how to view an ome.zarr file stored in S3 using the User Interface
how to view an ome.zarr file stored in S3 using the scripting editor of Fiji.
Setup
Install Fiji on a local machine.
BigDataViewer is already included in Fiji.
To install MoBIE, go to
Help>Update...In the ImageJ updater dialog, click on
Manage update sites.The MoBIE` should already be listed. Select it and click
Close.Click
Apply changesto install it.- If MoBIE` is not listed:
Click
Add update site.Enter for the name
MoBIE(so you can identify it) and for the URL:https://sites.imagej.net/MoBIE/. See How to install an update site.Click
Apply changesto install it.
Restart Fiji.
Resources
Samples images from the Image Data Resource (IDR) that have been converted into https://github.com/ome/ngff. A list of available files can be found here.
- Script: Groovy script for opening the images in BigDataViewer using MoBIE.
Watch an introductory video.
Step-by-step
Opening an ome.zarr file in BigDataViewer
Launch Fiji.
Go to Plugins > BigDataViewer > HDF5/N5/Zarr/OME-NGFF Viewer
A dialog pops up.
In the text field, you can click Browse to open local data or enter the desired URL e.g.
https://uk1s3.embassy.ebi.ac.uk/idr/zarr/v0.4/idr0062A/6001240.zarr
If you Click the OK` button tbis will open the image in BigDataViewer. You can also click the Detect datasets button to inspect the content of the zarr file, such as individual pyramid arrays and labels. You can select multiple items, for example to open the (multiscales) image under labels alongside the parent (multiscales) image.
When the image is displayed in the BigDataViewer, select the dialog and press P to display the rendering controls.
Modify the settings as you see fit.
Opening an ome.zarr file in MoBIE
Launch Fiji.
Go to Plugins > MoBIE > Open > Open OME-Zarr…
A dialog pops up.
In the text field, enter the desired URL e.g.
https://uk1s3.embassy.ebi.ac.uk/idr/zarr/v0.4/idr0062A/6001240.zarr
Click the OK button.